Do sisters ever suspect their brothers?

I love my little sister (14) more than anyone and we have a special bond..
Few days ago at dinner my eyes accidentally fell on a wrong place
I am quite sure she noticed that and was visibly uncomfortable momentarily
Thankfully she didn't act too indifferently.. but started talking less I think
Also for the last 2 days she skipped the usual hugs and kisses before heading to school (Usually she comes to my room in the morning)
Well if it's she like she is old enough to stop that, I am totally cool with it
But I am uncomfortable thinking whether I became a bad guy in her eyes? Is it appropriate to talk to her about these?
Do sisters ever suspect their brothers?
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