The phrase refers to motorcycle and car races. It would be pronounced at the start of a race, when the drivers were all lined up at the starting line, to signal the imminent start of the race. Ready, set, go. You can also see it at a stop light. It's something most men can't repress.
Do gentlemen really need to be told to get prepared? You can interpret this question any way you like, in a literal or figurative way.
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