What should I do? Should I end my marriage?

Background: Prior to my (m24) current partner (f25), I maintained an on/off relationship with someone who cheated on me from the get go. Needless to say, I had trust issues by the time I met current partner. When we first met, we agreed that during the talking stage, we weren't to sleep with anyone else. However, after we got more serious, my past began to kick in and I began to question if she had fooled around any during the talking stage. She adamantly denied it and was very offended when I questioned her. However, a few months later, I caught her in a lie about a story involving one of the guys she had been with, and gave her an ultimatum that she either tell me the truth or we're done. She finally told me she did sleep with someone 14 days into us talking, but regretted it and never did anything after. It was 14 days, so the sex part didn't really bother me. It was just that she lied about it for like seven months. Keeping my word, I forgave her for this and moved past it (never brought it up during any fights, even now).

Flashforward to now, 5 years later: I WFH and my wife is a stay at home mom for our two daughters. Being WFH, I've had the opportunity to be present for our children in ways my parents were never able to do. We had a great marriage for a long time. And then she became emotionally distant. Now granted, if she has cheated on me, it would be really hard. I never leave the house and she doesn't like to drive. The only time she would have to do it would be while I'm sleeping in on the weekends. However, when the distance began, my past and the lingering thought of what she did (still haven't brought it back up to her) before, I questioned her if she had cheated on me. She became very defensive and keep saying no, before eventually just going to bed and leaving me hanging. I don't know what to do. I feel like we're at a cross-roads. Am I just an insecure man with a bad past? Or is there some validity to this? Any advice is appreciated.

Updates
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Update: I want to add that we live with my wife's parents. My wife's mom has begun acting strange, and though my wife is very open with her phone, I fear she may be cheating through her mom's phone. The biggest reason is her mom's become super suspect. While having me fix something on her phone, I noticed that her mom's call logs, texts, and FB Messages were all deleted, except for the messages from me and my wife. Little shady, if you ask me.
What should I do? Should I end my marriage?
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